Molecular Survey of Theileria and Babesia Species in Small Ruminants and Ticks from Sivas Region of Turkey

Kürşat Altay, Ahmet Duran Ataş, Erkan Özkan

Abstract


This study was carried out to investigate the presence and distribution of Theileria and Babesia species with microscopic examination and reverse line blotting (RLB) techniques in sheep, goats and ticks from Sivas (Sivas, Kangal, Koyulhisar, Yıldızeli) of Central Anatolia region of Turkey. For this purpose 309 blood samples (206 sheep and 103 goats) and 478 tick samples that divided into 27 pools as the species were collected from sheep and goats. The total genomic DNAs were extracted from 309 blood samples and 27 tick pools were tested by RLB for Theileria and Babesia species. A total of 8 animals (2.59 %) were found to be positive for Theileria spp. piroplasms in microscopic examination of the smears. Four species or genotypes (Theileria ovis, Theileria sp. MK, Theileria sp. OT3, Babesia ovis) were detected in sheep, goats and ticks pools with RLB.  18S SSU Infection rate of T. ovis was 56.80 % in sheep, 3.88 % in goats and totally 39.16 %. Theileria sp. OT3 was detected in 4.85 %  of sheep and 3.24 % of all animals, while Theileria sp. MK was detected 3.88 % in sheep and 2.59 % in all animals. rRNA gene sequences were detected for all species or genotypes. The sequences were submitted to GenBank under accession numbers; KF266694, KF266695, KF434321 and KF440691. The tick species were identified as Rhipicephalus bursa, Haemaphysalis sulcata, Hyolamma marginatum, H. detritum and Dermacentor marginatus. According to the result of tick pools analysed by RLB, T. ovis were detected in R. bursa, H. marginatum, H. detritum and Hae. sulcata while B. ovis were determined in R. bursa.


Keywords


Theileria, Babesia, sheep, goat, tick, RLB, Sivas

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References


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