Çerezlik Kabak (Cucurbita pepo L.) Hatlarının SSR (Simple Sequence Repeat) Markörleri ile Karakterizasyonu

Necibe Kayak, Önder Türkmen, Ali Tevfik Uncu, Yeşim Dal

Abstract


This study was carried out to determine the molecular characterization of 83 well-established lines in the prospective S4 stage in the edible pumpkins (Cucurbita pepo L.). The molecular diversity among the pumpkins genotypes was determined by SSR markers. Thirty one EST- SSR markers yielded a total of 122 amplified DNA fragments, of which 107 (87.70%) were polymorphic, were used to measure the degree of genetic diversity. The DARwin software was used to generate a Dice coefficient dissimilarity matrix that was then used to construct an unweighted neighbor-joining dendrogram of the accessions. Neighbor-joining dendrogram demonstrated that the germplasm collection is divided into three main groups. The highest PIC value was calculated for the marker CUTC006209 as 0.42. According to the Mantel test results, correlation of the dissimilarity matrix and the dendrogram was 0.99 which is a very high score that indicates a very strong degree of correlation. Genetic relationships calculated based on molecular genetic data demonstrated the degree of diversity within the population along with the pattern of clustering among accessions which will provide valuable information to breeders for future breeding programs.


Keywords


Edible pupkins, molecular description, SSR

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